纯度

> 95 % as determined by SDS-PAGE

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物活性

1. Measured by its binding ability in a functional ELISA. Immobilized mouse KITL at 2 μg/ml (100 μl/well) can bind mouse CD117 with a linear range of 1.28-32 ng/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 5-30 ng/mL.

蛋白构建

A DNA sequence encoding the amino acids (Met 1-Ala 189) of mouse KITL (P20826-1) was fused with a polyhistidine tag at the C-terminus

表达宿主

Baculovirus-Insect Cells

种属

Mouse

预测 N 端

Lys 26

分子量

The secreted recombinant mouse KITL consists of 175 amino acids and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 7.4
Please contact us  for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.