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Sigma|C4405|9028-67-5|Choline Oxidase from Arthrobacter glob
  • 英文名称:Choline Oxidase from Arthrobacter globiformis
  • 品牌:Sigma
  • 产地:进口
  • 货号:C4405
  • cas:9028-67-5
  • 价格: ¥1521.00/瓶
  • 发布日期: 2018-05-14
  • 更新日期: 2024-11-20
产品详请
产地 进口
品牌 Sigma
货号 C4405
英文名称 Choline Oxidase from Arthrobacter globiformis
包装规格 100UN/500UN
纯度 %
CAS编号 9028-67-5
别名 Choline: oxygen 1-oxidoreductase
分子式

客户专员:刘小姐


产品名称:Sigma|C4405|9028-67-5|Choline Oxidase from Arthrobacter globiformis

产品描述:

包装

100, 500 units in poly bottle

Unit Definition

One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.

Application

Choline oxidase from Sigma has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection.[3]

Biochem/physiol Actions

Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the investigation of the reaction mechanism for the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine.[1] The enzyme is a flavoprotein with a molecular weight of approximately 83,000 Da according to gel filtration or approximately 71,000 Da according to SDS gel electrophoresis. The optimum pH is found to be around pH 7.5 and the isoelectric point (pI) around pH 4.5

性质:

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Analytical and Industrial Enzymes, Cholinesterase and Organophosphate Detection Reagents, Coupling Enzymes,

Enzyme Class Index, General Metabolic Enzymes, General Metabolic Enzymes A-H, 代谢组学, 生化试剂, 细胞生物学, 酶、抑制剂和底物

Less...

form          lyophilized powder

storage temp.   ?20°C

    

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