客户专员：刘小姐

产品名称：Sigma|H4272|37326-33-3|透明质酸酶 来源于牛睾丸|Hyaluronidase from bovine testes

产品描述：

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。

Unit Definition

One unit will cause a change in A600 of 0.330 per minute at pH 5.35 at 37°C in a 2.0 ml reaction mixture (45 minute assay).

Application

Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass from newly ovulated eggs, when preparing the eggs for in vitro culture.

Reconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml.

Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.

性质：

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Carbohydrate Hydrolysis, Carbohydrate hydrolysis & PTM analysis,

Complex Carbohydrate Analysis, Enzyme Class Index, Hyaluronidase, Hyaluronidase for Hyaluronic Acid Hydrolysis, Mouse Embryo Culture, Reagents and Media Supplements, 特殊培养基系统, 生化试剂, 细胞培养, 酶、抑制剂和底物

Less...

sterility   aseptically processed

type          Type IV-S

form          powder

mol wt   mol wt ~55 kDa (four subunits of 14 kDa each)

composition   Protein, 80-105% biuret

suitability   mouse embryo tested

storage temp.   ?20°C

1、产品质量问题，在产品售出7日内提出异议并且产品未有损坏，试剂类产品，需要提供详细的检测数据，创赛负责免费退换货服务；

2、本产品属于消耗品，不提供质保服务；

3、所有产品售后问题，请直接联系我司，我们将会尽快为您响应处理

关 键 词：科学仪器，Sigma，H4272，37326-33-3，透明质酸酶 来源于牛睾丸，Hyaluronidase from bovine testes，创赛品质，实验室耗材，生化试剂，创赛中国,原料药，大包装产品

CANSPEC品质提供的Sigma，H4272，37326-33-3，透明质酸酶 来源于牛睾丸，Hyaluronidase from bovine testes，创赛品质，性能*，其优秀的品质满足您不同实验的多种需求。

本产品由上海创赛品质提供，欢迎咨询全国免费服务