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产品名称：Sigma|F9252|福林酚溶液|Folin & Ciocalteu’s phenol reagent

产品描述：

Other Notes

Folin & Ciocalteu′s phenol reagent does not contain phenol. Rather, the reagent will react with phenols and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically.

Linkage

This item is also available as part of a kit.

包装

1 L in glass bottle

100, 500 mL in glass bottle

Application

Folin & Ciocalteu’s phenol reagent has been used for the estimation of total phenolic content in samples.

General description

Folin & Ciocalteu′s phenol reagent is most commonly used in the Lowry method for determining protein concentration.[7][6] It has also been used for the quantification of total phenolics.[10] In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution

stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750nm) or shoulder (660nm) are used to quantitate protein concentrations between 1-100 mg/ml while absorbance at 550nm is used to quantitate higher protein concentrations.

In the absence of copper, color intensity is determined primarily by the tyrosine and tryptophan content of the protein, and to a lesser extent by cysteine and histidine. Copper(II) has no effect on color formation by tyrosine, tryptophan, or histidine, but reduces color formation due to cysteine.[7][2][8][4][9]

Many modifications of the original assay procedure have been published,[2] including methods for enhancing the color development,[4][3] for determining the content of insoluble proteins,[7][1] and for automating the procedure.[5] Compounds including many buffers, chelating agents, detergents, and cyclic organic compounds can interfere with the Lowry protein assay.[2]

Folin & Ciocalteu′s phenol reagent can also be used as a spray reagent in chromatographic procedures.

性质：

Related Categories Core Bioreagents, Enzyme Substrates, General Protein Quantitation, Protease Detection Kits, Quantitation Kits and Reagents,

Research Essentials, Stains and Dyes, 分子生物学, 生化试剂, 蛋白质定量, 蛋白质组学, 酶、抑制剂和底物

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concentration   2 N

suitability   suitable for determination of total proteinby Lowry method

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