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产品名称：Sigma|C6885|9001-12-1|胶原蛋白酶 来源于溶组织梭菌|Collagenase from Clostridium histolyticum

产品描述：

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Collagenase is activated by four gram atom calcium, per mole enzyme. It is inhibited by ethylene glycol-bis(β-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, β-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.Collagenase enzymes and neutral protease plays an important role in the effective release of cells from tissue.[2] Collagenase recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often a neutral amino acid.[4]

Caution

As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.

包装

100, 500 mg in poly bottle

Preparation Note

Also contains clostripain, nonspecific neutral protease, and tryptic activities.

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Application

Collagenase may be used: [3][1]

? for the preparation of arterial tissue for the study of advanced glycosylation end products (AGE)

? for use along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues.

? in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes

? for the preparation of viable hepatocytes from rat liver and for the isolation of fat cells from rat adipose tissue

General description

Clostridium histolyticum is a pathogenic clostridium that produces collagenase. This is a mixture of enzymes containing collagenase, non-specific proteases and clostripain

性质：

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Cell Dissociation,

Cell Dissociation and Cell Lysis, Cell Signaling Enzymes, Collagenases, Core Bioreagents, Core Bioreagents Enzymes, Crude Collagenase, Use-Tested, Cytoskeleton and Extracellular Matrix, Enzyme Class Index, Enzymes for Diagnostic Kit Manufacturing, Extracellular Matrix, Extracellular Matrix Peptides and Proteins, Histology, MMP Enzymes, Matrix Metalloproteinases Products, Other Apoptosis Related Reagents, Other Apoptosis Related Reagents Enzymes, Proteases, Proteases & Protein Sequencing, Proteolytic Enzymes, Proteolytic Enzymes and Substrates, Research Essentials, 生化试剂, 细胞信号转导和神经科学, 细胞生物学, 酶、抑制剂和底物

Less...

form                  powder

suitability          release of physiologically active                           rat epididymal adipocytes tested

Featured Industry   Diagnostic Assay Manufacturing

shipped in          wet ice

storage temp.          ?20°C

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