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产品名称：Sigma|C1345|9001-05-2|过氧化氢酶 来源于牛*|Catalase from bovine liver

产品描述：

Components

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Application

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver is used for the following applications:

? Gel filtration[5]

? Analytical filtration on Ultrogel and glycerol gradient centrifugation[1]

? Size exclusion chromatography (HPLC)-HPLC analysis of estrogen receptor[2]

? Enzyme assays[3][6]

? Alcohol oxidase activity[4]

Biochem/physiol Actions

This product doesn not need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

激活过氧化氢的分解，用于研究活性氧在基因表达和细胞凋亡中的作用的天然抗氧化剂。

Caution

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

包装

1, 10, 100 g in poly bottle

Preparation Note

This product is a powder suitable for cell culture with activity at 2,000-5,000 units/mg. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

Unit Definition

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

General description

CAT (catalase) is an endogenous antioxidant enzyme, thus conferring protection to cells against damage by ROS (reactive oxygen species). In humans, this gene is localized to chromosome 11p13, which is composed of 12 introns and 13 exons.[7]

性质：

Related Categories 1.11.1.6 Catalase, 1.x.x.x Oxidoreductases, Application Index, Cell Dissociation, Cell Dissociation (Cell Culture Tested),

Cell Dissociation Enzymes, Cell Dissociation and Cell Lysis, Enzyme Class Index, 生化试剂, 细胞培养, 试剂和添加剂, 酶、抑制剂和底物

Less...

form                  powder

mol wt          tetramer mol wt ~250 kDa

isoelectric point   5.4

suitability          suitable for cell culture

shipped in          wet ice

storage temp.          ?20°C

Gene Information   cow ... CAT(280743)

1、产品质量问题，在产品售出7日内提出异议并且产品未有损坏，试剂类产品，需要提供详细的检测数据，创赛负责免费退换货服务；

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