PE Mouse Anti-Human CD146

Purified Rat Anti-Mouse CD107b

Product Information

Material Number: 550292

Alternate Name: CD107b; Lamp2; LAMP-2; Lysosome-associated membrane glycoprotein 2; LGP-B

Size: 1 mL

Concentration: 31.25 μg/ml

Clone: M3/84

Immunogen: Mouse C57Bl/6 peritoneal exudate cells

Isotype: Rat (LEW x BN) IgG1, κ

Reactivity: QC Testing: Mouse

Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium 

azide.

Description

The M3/84 monoclonal antibody specifically binds to CD107b which is also known as Mac-3, Lysosome-associated membrane protein 2 

(LAMP-2/Lamp2/Lamp II), and Lysosomal membrane glycoprotein type B (LGP-B). CD107b is a single-pass type I transmembrane 

glycoprotein that constitutes a major integral membrane protein of lysosomes and may play a role in lysosomal function. CD107b is also 

expressed on the surface of mouse mononuclear phagocytes. Surface expression of the 92-110-kDa glycoprotein antigen increases during 

differentiation of monocytes to activated macrophages and may play a role in adhesion. The M3/84 mAb can detect CD107b antigen on tissue 

macrophages, thioglycollate-elicited peritoneal macrophages, and some myeloid cell lines, but not on lymphocytes or monocytes. In the bone 

marrow, very few cells display CD107b antigen on the surface, but a large proportion express cytoplasmic CD107b. The M3/84 antibody has 

also been reported to stain dendritic cells and endothelium in sections of thymus (both medulla and cortex), lymph nodes, spleen (white pulp), 

and gut-associated lymphoid tissue.

Preparation and Storage

Store undiluted at 4°C.

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography

Application Notes

Application

Flow cytometry                                 Routinely Tested

Immunohistochemistry-paraffin            Tested During Development

Immunohistochemistry-zinc-fixed          Tested During Development

Immunohistochemistry-frozen               Reported

Immunoprecipitation                           Reported

Suggested Companion Products

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559072                    Purified Rat IgG1, κ Isotype Control        0.25 mg      R3-34

550325                    Biotin Mouse Anti-Rat IgG1/2a               1 mL           G28-5

550880                    DAB Substrate Kit                                500 Tests    (none)

550946                    Streptavidin HRP                                  50 mL         (none)

559148                    Antibody Diluent for IHC                       125 mL        (none)

550523                    IHC Zinc Fixative                                  1000 mL       (none)

Product Notices

 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

 2. An isotype control should be used at the same concentration as the antibody of interest.

 3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before 

discarding to avoid accumulation of potentially explosive deposits in plumbing.

 4. Source of all serum proteins is from USDA inspected abattoirs located in the United States