APC Mouse Anti-Human CD117

Product Information

Material Number: 550412

Alternate Name: KIT ; c-Kit; SCFR; PBT: Mast/stem cell growth factor receptor

Size: 0.1 mg

Concentration: 0.2 mg/ml

Clone: YB5.B8

Isotype: Mouse IgG1, κ

Reactivity: QC Testing: Human

Workshop: V C009

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Description

The YB5.B8 monoclonal antibody specifically binds to CD117. CD117, also known as c-Kit, is a 145 kDa cell-surface glycoprotein with tyrosine kinase activity. CD117 is present on hematopoietic progenitor cell subsets, thymocytes, mast cells, hepatocytes and histiocytes. CD117 serves as a cytokine receptor for steel factor (SLF), also known as stem cell factor (SCF) or mast cell growth factor (MGF). The interaction of c-Kit and SLF is crucial to hematopoiesis, mast cell differentiation, melanogenesis, and germ cell development. The ability of the YB5.B8 antibody to block the binding of c-Kit ligand is still controversial.

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Application Notes

Application

Flow cytometry              Routinely Tested

Suggested Companion Products

Catalog Number      Name                                         Size         Clone

555751                 APC Mouse IgG1, κ Isotype Control 100 tests MOPC-21

Product Notices

 1. Since applications vary, each investigator should titrate the reagent to obtain optimal