Purified NA/LE Rat Anti-Mouse CD127
Product Information
Material Number: 550426
Alternate Name: IL-7 Receptor α chain
Size: 0.5 mg
Concentration: 1.0 mg/ml
Clone: SB/14
Immunogen: BALB/c mouse pre-B cell line 1A9
Isotype: Rat IgG2a, κ
Reactivity: QC Testing: Mouse
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 
0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of 
protein as determined by the LAL assay.
Description
The SB/14 monoclonal antibody reacts with CD127, the 65-75-kDa type-I transmembrane protein IL-7Rα. The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7Rα and CD132, the common γ chain. CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily). Interaction between IL-7 and its receptor is important for the proliferation of pre-B lymphocytes and can also trigger proliferation of CD4-CD8- immature thymocytes, as well as mature T cells in the periphery. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities. IL-7Rα is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density γδ TCR upregulate CD127 expression in response to IL-2, which may be secreted by nei*oring αβ TCR-bearing T cells. The SB/14 mAb neutralizes IL-7-induced proliferation of the IL-7-dependent immature B-lymphocyte line 2E8 (detected by BrdU incorporation).

Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Application Notes
Application
Flow cytometry                       Routinely Tested
Blocking                                 Reported
Immunohistochemistry             Not Recommended
Immunoprecipitation                Not Recommended
Western blot                          Not Recommended
Suggested Companion Products
Catalog Number  Name                                                   Size      Clone
553926             Purified NA/LE Rat IgG2a κ Isotype Control 0.5 mg R35-95
559619             BrdU Flow Kit- (FITC) Part B                     80 tests (none)
554061             PE Streptavidin                                       0.5 mg (none)
Product Notices
 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.