APC Annexin V

Product Information

Material Number: 550475

Size: 200 tests

Vol. per Test: 5 μl

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Description

Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The 

apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, 

condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features. 

In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma 

membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding 

protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including APC. 

This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing 

apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, APC Annexin V staining can identify apoptosis at an earlier 

stage than assays based on nuclear changes such as DNA fragmentation

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Application Notes

Application

Flow cytometry               Routinely Tested

Suggested Companion Products

Catalog Number Name                                                    Size     Clone

559925            7-AAD                                                   2.0 ml  (none)

556454            Annexin V Binding Buffer, 10X concentrate 50 ml   (none)

Product Notices

 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

 2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before 

discarding to avoid accumulation of potentially explosive deposits in plumbing.

 3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

 4. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.

, or red diode laser.