Description

The MECA-32 antibody reacts with a dimer of 50-55-kDa subunits expressed on most or all endothelial cells in the embryonic and adult mouse, with the exception of cardiac and skeletal muscle and the brain. Normally in skeletal and cardiac muscle, MECA-32 antigen expression is limited to small arterioles and venules; however, under conditions of inflammation, it can be induced on previously non-expressing vessels in cardiac muscle. In the central nervous system (CNS), the panendothelial cell antigen expression is developmentally regulated. During embryonic development, the antigen is found on brain vasculature up to day 16 of gestation, after which it disappears. The cessation of MECA-32 antigen expression in the CNS may be associated with the establishment of the blood-brain barrier, which begins on day 16 of gestation. In the adult mouse, inflammation in the CNS can lead to re-expression of the panendothelial cell antigen.

Preparation and Storage

Store undiluted at 4°C.

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Application Notes

Application

Flow cytometry                      Routinely Tested

Immunohistochemistry-frozen   Tested During Development

Immunohistochemistry-paraffin  Not Recommended

Suggested Companion Products

Catalog Number Name                                          Size        Clone

559286            Biotin Goat Anti-Rat Ig                   0.5 mg    Polyclonal

559073            Purified Rat IgG2a κ Isotype Control 0.25 mg  R35-95

550880            DAB Substrate Kit                          500 tests (none)

550946            Streptavidin HRP                            50 ml      (none)

559148            Antibody Diluent for IHC                 125 ml     (none)

Product Notices

 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

 2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before 

discarding to avoid accumulation of potentially explosive deposits in plumbing.

 3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

 4. An isotype control should be used at the same concentration as the antibody of interest.

 5. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not 

performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.