Catalog Number: C22-672-01 Source:Escherichia coli.

Molecular Weight:Approximately 12.6 kDa, a single non-glycosylated polypeptide chain containing 119 amino acids.
Quantity:20μg/100μg/1000μg
AA Sequence: MGGWSSKPRQGMGTNLSVPNPLGFFPDHQLDPAFGANSNNPDWDFNPNKDHWPEAHQVG
AGAFGPGFTPPHGGLLGWSPQAQGILTTVPVAPPPASTNRQSGRQPTPISPPLRDSHPQA
Purity:>95% by SDS-PAGE and HPLC analyses.
Applications:
1. Immunochromatography (capture and conjugate);
2. Preparing monoclonal or polyclonal antibodies for HBsAg-preS1;
3. ELISA.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH 7.4, 50mM NaCl.
Endotoxin: Less than 1EU/μg of rHBsAg-preS1 as determined by LAL method.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage: T This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
-70°C. Avoid repeated freeze/thaw cycles.

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